Spore swabs are hardy and are not temperamental when environmental conditions change during international shipping or being mailed across the country during a heat wave for example. The best way to prove a mushroom sample is an aggressive isolation is by running the spore swab on an agar plate and adding the first or second transfer to a one pound grain bag to verify a proper yield. Some the best mushroom growers in the world use spore swabs to store isolated variety’s. It is important to note even when storing mushroom spore swabs refrigeration is the best practice for genetic maintenance. ![]() The main reason you want to maintain your genetics with a digital wine fridge is because you will never have to worry about unstable conditions, if a culture or cell gets freezer burnt it will cause the cells to “lyse” or break and degrade your genetics or worse make them inactive and unable to colonize completely. You’ll use millions of mushroom spores in this syringe, floating in the sterilized water. Once the material reaches room temperature you can inoculate your grain spawn or all in one grow kit. A spore syringe is a syringe with mushroom spores and sterilized water. Spore swabs, culture syringes should be kept in the refrigerator until 24 hours prior to use. This goes for both spores and liquid culture. the reason for using them soon is that spore prints can contain things other than mushroom spores due to the the fact that mushrooms do not grow in perfectly sterile conditions, and anything you do to destroy bacteria or other spores may harm the mushroom spores. A syringe has millions of mushroom spores that float in a sterile. Syringes should be used within a month or two, but have been known to last 6 months or more. ![]() Low nutrient recipes will generally survive the longest, by adding a minimal amount of food source will slow down the growth of the culture (1 gram light malt extract per liter).Īpproximately 39-41 degrees Fahrenheit is what I found to be the most effective range for long term storage ( 4-5 plus years). Spore syringes are commonly utilized mediums that house spores from any mushroom strain. A liquid culture syringe can last for multiple years in the refrigerator if the genetics are stored in the proper medium.
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